WebAfter cation exchange chromatography on a Toyopak IC-SP S cartridge, catecholamines and isoproterenol (internal standard) in urine samples were converted into the corresponding … WebSPE was performed on TOYOPAK IC-SP M containing propylsulfonic groups. Determination of PEP and EP was carried out using ion-pair reversed-phase HPLC with sodium dodecyl …

Assay for tyrosine hydroxylase by high-performance liquid ...

Webpassed through a Toyopak IC-SP M (H+ form, Tosoh, Tokyo, Japan) and concentrated five times by centrifugal evaporation. Aliquots (50 J.lI) of reaction products released were separated on a A-2H+ column (10 x 300 mm, Bio-Rad, CA, USA) at 85°C using 5 mM sulfuric acid as eluent at a flow rate of 0.3 mJ/min, monitored by an refractive index ... Web水路部技報 Vol. 20. 2002 GC/MSを用いた海底堆積物中における有機スズ化合物の定量法の検討 清水潤子 海洋汚染調主室 business plan for students pdf

Assay for dopamine β-hydroxylase in human plasma and …

WebApr 10, 1987 · A sensitive assay method for tyrosine hydroxylase in rat brain and adrenal medulla by high-performance liquid chromatography with fluorescence detection is described. L-DOPA formed enzymatically from the substrate L-tyrosine and alpha-methyldopa (internal standard), after clean-up with small cartrid … WebApr 10, 1987 · A sensitive assay method for tyrosine hydroxylase in rat brain and adrenal medulla by high-performance liquid chromatography with fluorescence detection is … WebSep 2, 1994 · Purification of fluorescent adducts with a Toyopak IC-SP S cartridge The reaction mixture of the NBD-ED adducts with carnitine and acylcarnitines diluted with 9 volumes of 10 mM HCl in methanol-water (4:1, v/v) was applied to a Toyopak IC-SP S cartridge, the cartridge was washed with 5 ml of 10 mM HCl-methanol (1:1, v/v) and the … business plan for steel manufacturing company