Tīmeklis2014. gada 16. apr. · Here, we report the synthesis and application of a cell permeable, fluorescent small molecule (SR101-F-araNMN) that can covalently label … CD38 protein was purchased from Sino biological (Cat. No: 10818-H08H); Darzalex (Daratumumab) was purchased from Janssen Biotech; Human IgG Isotype control (Cat. No.: 02–7102) and HRP conjugated Goat anti-Human IgG (H + L) Secondary Antibody (Cat. No.: # A18805, dilution for ELISA: 1:2000) FROM … Skatīt vairāk Human embryonic kidney epithelial cells 293 T (ATCC® CRL-3216™), B Lymphocyte Raji (ATCC®CCL-86™) and B Lymphoblast … Skatīt vairāk ELISA plates were coated with antigen (e.g. 2 μg/mL CD38 protein) at 4 °C overnight and then blocked with 1% BSA in PBS containing … Skatīt vairāk Anti-CD38 Antibody 3G3 were generated by immunization of mice with recombinant CD38 protein. After isolation of mouse splenocytes and … Skatīt vairāk ADCC activities of the antibodies were measured by DELFIA EuTDA Cytotoxicity Reagents (Perkin Elmer) according to the manufacturer’s instructions. Briefly, the tumor cells (target … Skatīt vairāk

Antigen-induced clustering of surface CD38 and recruitment of ...

TīmeklisNational Center for Biotechnology Information Tīmeklis2024. gada 13. nov. · Of note, in those cell lines that were responsive to daratumumab in CDC assays (>50% tumor cell lysis), CDC activity of HexaBody-CD38 was superior to daratumumab, with IC50 values for HexaBody-CD38 2.4- to 13-fold lower than for daratumumab. Moreover, HexaBody-CD38 unlocked CDC activity in 17 out of 28 … dick wilson actor

3G3 bound to the membrane CD38 to kill Raji cells by ADCC. a …

TīmeklisThe HiBiT Target Cell Killing (TCK) Bioassay platform enables highly sensitive and specific measurement of target cell killing induced by a variety of biologic drugs, including monoclonal antibodies (mAbs), bispecific antibodies and CAR-T cells. The assay is simple and homogeneous. TīmeklisIt bound to the antigen CD38 on a dose-dependent manner both by ELISA (Fig. 1a) and flow cytometry in Raji cells (Fig. 1b and c); meanwhile, 3G3 showed satisfactory cytotoxicity function on a dose ... Tīmeklis2015. gada 15. maijs · Similarly, experiments with isogenic MM cell lines expressing different levels of CD38 revealed that the level of CD38 expression is an important … dick wimberly