Phospho acc
WebAug 1, 2006 · The phosphorylation of AMPK and its downstream target, ACC, was assessed by using immunoblots with phospho-Thr-172 AMPKα (pAMPK) and phospho-Ser-79 ACC1 (Ser221 ACC2; pACC) antibodies. The levels of phosphorylated AMPK and the sum of phosphorylated ACC1 and ACC2 were normalized to endogenous AMPKα levels and … WebSep 1, 2024 · The Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to ACACA, and ACACB. This antibody recognizes Human, Mouse, and Rat antigen. The Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb Antibody has been validated for the following applications: …
Phospho acc
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WebAug 16, 2014 · This was accompanied with inactivation of acetyl-CoA carboxylase (ACC), as evidenced by the increased phosphorylation levels at Ser 79. In addition, PSP treatment also sensitized cancer cells toward γ-T3-induced cytotoxicity. ... (Thr172) (1:100) and anti-phospho-ACC (Ser79) (Cell Signal Technology) was applied overnight in a humidity … WebHowever, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). Castle, J.C. et al. (2009) PLoS One 4, e4369.
WebMay 17, 2013 · AMPK activation was reflected by phosphorylation of AMPKα (Thr 172) and ACC (Acetyl-CoA Carboxylase, Ser 79) , while MAPK activation was demonstrated by phosphorylation of JNK, ERK and p38 ... phospho- and non-phospho- AMPK, ACC and eIF2α were detected as described above (E); (B) ... WebACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the cytoplasm of most …
WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) Research with confidence – … WebApr 11, 2024 · A) Representative immunoblotting of ACC, phospho-ACC and Fas protein, GAPDH was used as loading control. B) Densitometry analysis of ACC shows similar protein levels in all experimental groups. C) Densitometry analysis of phospho-ACC shows an increase in Ser79 phosphorylation site in the offspring of MO+Epi group (a P < 0.001 vs …
WebIncreased expression of phospho-acetyl-CoA carboxylase protein is an independent prognostic factor for human gastric cancer without lymph node metastasis. Upregulation …
WebPhospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb 11818 20 µl 280 kDa Rabbit IgG Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 3676 20 µl 280 kDa Rabbit IgG Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat See www.cellsignal.com for individual component applications, species cross-reactivity, dilutions and ira wallace dighton ksWebLysates from F4 to F1 granulosa and theca cells were prepared and subjected to Western blotting using anti-phospho-AMPKα-Thr172, anti-AMPKα1/2 (α-pan AMPK antibodies … orchies cpWebACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and ... orchies crematoriumWebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein. Background Acetyl-CoA carboxylase … ira wallace booksWebThe HTRF Total ACC cellular assay monitors the expression levels of both ACC 1 and ACC2, and can be used as a normalization assay with the HTRF phospho-ACC Ser79 kit. This kit is compatible with the buffers from the phospho-ACC kit, so the same lysate can be used for analyzing both the phosphorylated and the total protein populations. orchies ehpadWebIn these experiments using a phosphoserine antibody to ACC and a phosphothreonine antibody to AMPK, evidence was obtained for phosphorylation and activation of ACC in … ira wallach writerWebACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and ... orchies festimania